Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 80, Issue 1, Pages 304-316Publisher
WILEY
DOI: 10.1002/mrm.27018
Keywords
MRI; cancer; cell tracking; CTLs; Tregs; MDSCs; vaccines; DepoVax
Funding
- Nova Scotia Health Research Foundation
- IWK Health Centre
- Beatrice Hunter Cancer Research Institute Cancer Research Training Program (CRTP)
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PurposeMRI cell tracking can be used to monitor immune cells involved in the immunotherapy response, providing insight into the mechanism of action, temporal progression of tumor growth, and individual potency of therapies. To evaluate whether MRI could be used to track immune cell populations in response to immunotherapy, CD8(+) cytotoxic T cells, CD4(+)CD25(+)FoxP3(+) regulatory T cells, and myeloid-derived suppressor cells were labeled with superparamagnetic iron oxide particles. MethodsSuperparamagnetic iron oxide-labeled cells were injected into mice (one cell type/mouse) implanted with a human papillomavirus-based cervical cancer model. Half of these mice were also vaccinated with DepoVax(TM) (ImmunoVaccine, Inc., Halifax, Nova Scotia, Canada), a lipid-based vaccine platform that was developed to enhance the potency of peptide-based vaccines. ResultsMRI visualization of CD8(+) cytotoxic T cells, regulatory T cells, and myeloid-derived suppressor cells was apparent 24h post-injection, with hypointensities due to iron-labeled cells clearing approximately 72h post-injection. Vaccination resulted in increased recruitment of CD8(+) cytotoxic T cells, and decreased recruitment of myeloid-derived suppressor cells and regulatory T cells to the tumor. We also found that myeloid-derived suppressor cell and regulatory T cell recruitment were positively correlated with final tumor volume. ConclusionThis type of analysis can be used to noninvasively study changes in immune cell recruitment in individual mice over time, potentially allowing improved application and combination of immunotherapies. Magn Reson Med 80:304-316, 2018. (c) 2017 International Society for Magnetic Resonance in Medicine.
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