Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 17, Issue 15, Pages 3732-3736Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9ob00408d
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Funding
- Department of Biotechnology, the Government of India [BT/PR23254/MED/122/88/2017]
- SERB, Government of India [EMR/2016/005768]
- CSIR, New Delhi
- IISER Bhopal
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The endoplasmic reticulum (ER) constitutes about half of the total membrane of a eukaryotic cell, and defects in the ER have been shown to be linked with a variety of diseases. To investigate these underlying mechanisms in detail, the specific labelling of the ER for high-resolution long-term live-imaging can serve as an important tool. Here, we report the identification of a stimulated emission depletion (STED) microscopy-compatible BODIPY derivative (NH2-BODIPY) to selectively image the ER. In contrast to the conventional ER-Tracker (TM) dye, NH2-BODIPY selectively labels the ER at a much lower concentration with no detectable cytotoxicity. Conventional imaging agents are often unstable under the intense light field used for STED microscopy, but NH2-BODIPY is stable due to its robust structure and therefore it has the potential to be widely exploited for ER imaging.
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