Journal
MABS
Volume 9, Issue 4, Pages 595-602Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/19420862.2017.1304341
Keywords
Aggrecanase; cartilage; endocytosis; extracellular matrix; proteoglycanase
Categories
Funding
- Arthritis Research UK [20563]
- Arthritis Research UK Center for Osteoarthritis Pathogenesis [20205]
- Cancer Research UK [C100/A8243]
- Kennedy Trust for Rheumatology Research
- orthopedic Research UK [483]
- National Institute of Arthritis and Musculoskeletal and Skin Diseases [AR40994]
- Versus Arthritis [20563] Funding Source: researchfish
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The potent aggrecanase ADAMTS-5 is constitutively secreted by chondrocytes, but it is rapidly endocytosed in normal cartilage via the cell surface endocytic receptor LRP1. Therefore it is difficult to detect the total ADAMTS-5 activity produced. In this study, we isolated a monoclonal anti-ADAMTS-5 antibody 1B7 that blocks LRP1-mediated internalization without affecting the aggrecanolytic activity. Addition of 1B7 to cultured human chondrocytes revealed the full aggrecanolytic activity of ADAMTS-5 generated by the cells. 1B7 is a useful tool to estimate the ADAMTS-5 activity and to identify its potential roles in the tissues.
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