Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 86, Issue -, Pages 227-232Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2017.08.009
Keywords
Human gastrointestinal model ARIS; Propionic acid; Foodborne pathogens; HT-29 cells
Categories
Funding
- CIATEJ (Mexico) (bilateral agreement Brazil-Mexico)
- CNPq [312336/2014-4]
- CAPES [PNPD 2469/2011]
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The survival of bacterial strains isolated from tejuino was evaluated in the human gastrointestinal model (ARIS) using saline solution and simulated food as matrices. Antagonistic activity towards pathogenic bacteria, short-chain fatty acids production and adhesion to HT-29 cells were also studied. Leuconostoc citreum CIATEJ BI-49.1, Weissella cibaria CIATEJ Chryseobacterium bovis CIATEJ BI-55.1 and Bacillus safensis CIATEJ BI-75.1 were selected and inoculated in ARIS model. B. safensis and C bovis were unable to survive the simulated human gastrointestinal tract conditions. Leu. citreum seemed to be more resistant to gastrointestinal conditions than W. cibaria, recovering 7-8 log CFU/mL. In the antagonistic assay, Leu. citreum showed the highest (p < 0.05) inhibition towards S. aureus and S. Typhimurium strains, while W. cibaria had the highest (p < 0.05) activity towards L monocytogenes. In regards of short chain fatty acids production, Leu. citreum showed highest (p < 0.05) production of formic, propionic and butyric acids (0.10, 37.52 and 2.65 g/L, respectively) and higher percentage of adhesion to HT-29 cells (approximately 25%) than W cibaria (approximately 0.5%). Leu. citreum could be a potential probiotic strain due to its tolerance to gastrointestinal conditions, antagonistic activity towards foodborne pathogens, short-chain fatty acids production and adhesion to HT-29 cells. (C) 2017 Elsevier Ltd. All rights reserved.
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