Journal
LEUKEMIA RESEARCH
Volume 52, Issue -, Pages 20-27Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.leukres.2016.11.002
Keywords
Myelodysplastic syndrome; Erythropoietin; Immune modulation; T cells; Lymphocytes
Categories
Funding
- Israel Cancer Association
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The immune system is impaired in myelodysplastic syndromes (MDS) and plays a role in the pathogenesis of the disease. Here we show effects of recombinant human erythropoietin (rHuEPO) on T cell (CD4(+), CD8(+) and CD4(+)CD25(+)) number and function in MDS patients. Healthy (20 subjects), MDS patients without rHuEPO treatment ('MDS', 13), and MDS patients treated with rHuEPO ('MDS+EPO', 17) were examined. CD4(+) and CD8(+) T cell numbers were reduced and increased respectively in MDS compared to healthy subjects. EPO treatment normalized these levels. CD4(+)CD25(+) cell numbers, lower in MDS, were normalized in MDS+EPO. In vitro activation of CD4(+) and CD8(+) cells with phytohemagglutinin as measured by CD69 expression, demonstrated a 7.2 fold increase in CD4(+) activation vs 13.6 fold for MDS and MDS+EPO respectively (p=0.004); and 10.2 fold (MDS) vs 18.6 fold (MDS+EPO, p < 0.003) for CD8(+) T cells. Expression of the co-stimulatory marker CD28, decreased in CD4(+) and CD8(+) T cells in MDS, was normalized in MDS+EPO CD4(+) T cells. Subgroup analysis of milder disease (WHO RA and RARS) and more advanced disease revealed no difference in CD4(+) and CD8(+) T cell numbers. However, the activation of these cells in the RA/RARS subgroup was impaired in EPO-untreated and enhanced in EPO-treated MDS patients. Our data suggest that EPO treatment improves immune abnormalities in MDS and may depend on disease severity. (C) 2016 Elsevier Ltd. All rights reserved.
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