Journal
LASERS IN MEDICAL SCIENCE
Volume 32, Issue 9, Pages 1959-1964Publisher
SPRINGER LONDON LTD
DOI: 10.1007/s10103-017-2292-9
Keywords
Leishmaniosis; PDT; Laser; Macrophage
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Funding
- Coordination for the Improvement of Higher Education Personnel-CAPES (Post-Doctoral research grant - SCPSO)
- National Council for Scientific and Technological Development-CNPq (MSci Grant - EJOT)
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The aim of this study is to evaluate the effects of ApPDT (antiparasitic photodynamic therapy) on the interaction of Leishmania braziliensis with J774 macrophages, used as a photosensitizer, methylene blue associated with red laser. The tests are in triplicate and the samples divided into four groups: control, photosensitizer, laser, and ApPDT. The photosensitizer used was the methylene blue at concentration of 12.5 mu g/mL. The parameters of the laser were lambda = 660 nm, 40 mW, and 8.4 J/cm(2). Samples are analyzed by optical microscopy through the identification and counting of infected and uninfected macrophages, parasite load, infectivity, and infection index. Statistical analysis used ANOVA test with Tukey post-test, being considered statistically significant p < 0.05. The analysis of the interaction tests shows that the infection rate in the ApPDT group in relation to the control group presents a statistically significant reduction (p < 0.0001) of 71% at both 24 and 48 h (p < 0.0001) of 62%. ApPDT reduces the number of macrophages infected by Leishmania braziliensis, as well as the number of intracellular parasites, being a possible alternative therapy in the treatment of cutaneous leishmaniosis.
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