Journal
CHEMICAL SCIENCE
Volume 10, Issue 15, Pages 4227-4235Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9sc00220k
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Funding
- National Key Basic Research Support Foundation of China [2015CB856301]
- National Scientific Foundation of China [21778002, 21571007, 21621061, 21527801]
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Time-resolved fluorescence lifetime imaging (FLIM) in the near-infrared region of 900-1700 nm not only allows a deep tissue penetration depth but also offers the unique benefit of the quantitative visualization of molecular events in vivo and is independent of local luminescence intensity and fluorophore concentration. Herein, we report the design of a wide-range pH sensitive molecular probe based on Yb3+ porphyrinate. The Yb3+ probe shows increasing NIR emission and lifetime with pK(a) values of ca. 6.6 from pH 9.0 and 5.0 and also displays an elongated lifetime from ca. 135 to 170 s at lower pH values (5.0-1.0) due to aggregation and reduced exposure to water at low pH values. Importantly, the probe is able to monitor a wide range of in vivo gastrointestinal pH values in mice models and the potential applications in imaging-guided gastrointestinal diagnostics and therapeutics were revealed. This study shows that lifetime contrast is important for preclinical imaging; lanthanide complexes could be successfully used in the design of stimuli-responsive NIR probes for advanced in vivo imaging.
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