Journal
ANALYST
Volume 144, Issue 8, Pages 2811-2819Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9an00153k
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Funding
- National Natural Science of China [61334008]
- Instrument Developing Project of the Chinese Academy of Sciences [YZ201402]
- National Key Research and Development Program of China [2018YFF0214900]
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We present an integrated microfluidic device for quantifying intracellular materials at the single-cell level. This device combines a dual-well structure and a microfluidic control system. The dual-well structure includes capture wells (20 m in diameter) for trapping a single cell and reaction wells (200 m in diameter) for confining reagents. The control system enables a programmable procedure for single-cell analysis. This device achieves highly efficient trapping of single cells, overcoming the Poisson distribution, while affording sufficient biochemical reagents for each isolated reactor. We successfully utilized the presented device to monitor the catalytic interaction between intracellular alkaline phosphatase enzyme and a fluorogenic substrate and to quantify the intracellular glucose concentration of a single K562 cell based on an external standard method. The results demonstrate the feasibility and convenience of our dual-well array microfluidic device as a practical single-cell research tool.
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