Influence of extraction methods of bay leaves (Syzygium polyanthum) on antioxidant and HMG-CoA Reductase inhibitory activity

Objective: Bay leaf, one of the plants in Indonesia that has been shown to have activities to reduce cholesterol in the blood. HMG-CoA Reductase inhibition is one of many mechanisms in lowering the level of cholesterol in the blood. Here, we reported the inhibitory activity of HMG-CoA Reductase of bay leaves ethanol extracts that we suspected to be the mechanism of action of bay leaves in reducing cholesterol in the blood. In this research we also investigated the correlation between the inhibitory activities, the total phenol content and antioxidant activities of bay leaves (Syzygium polianthum) ethanol extracts. Methods: The inhibitory activity of HMG-CoA Reductase was determined kinetically at 340 nm using simvastatin as positive control. In vitro scavenging assays of 2,2-dipheny1-1-picrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), and beta-carotene method were used to determine the antioxidant activities. The total phenolic content was determined by Folin-Ciocalteu's method. Results: The IC50 of bay leaves ethanolic extract obtained by percolation and soxhlet extraction method towards HMG-CoA Reductase enzyme activity were 49.50 +/- 0.700 mu g/mL and 15.50 +/- 0.707 mu g/mL, respectively, while the IC50 of simvastatin was 0.00238 +/- 0.00004 mu g/mL. The antioxidant activity and total phenolic content of bay leaves ethanolic extract obtained by Soxhlet extraction method was higher compared to the percolation method (DPPH and beta carotene assay results). The 3D linear analysis showed that there was a high correlation between the inhibition activities of HMG-CoA Reductase pattern of both extract types and the total phenol pattern and also the antioxidant pattern of these extracts. Conclusion: The result showed that the bay leaves ethanolic extract have a potent activity to reduce the cholesterol serum level by inhibition of HMG-CoA Reductase activity. The activity was due to the phenolic compounds in the extracts as well as the antioxidant activity of the extracts.