Journal
LAB ON A CHIP
Volume 17, Issue 13, Pages 2264-2271Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7lc00155j
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Funding
- DARPA [W911NF-12-2-0036]
- NIH [5R01EB020004-03]
- FDA [HHSF223201310079C]
- Wyss Institute for Biologically Inspired Engineering at Harvard University
- National Science Foundation under NSF [1541959]
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Trans-epithelial electrical resistance (TEER) is broadly used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured under static conditions in vitro, however, there is no standard methodology for its application to microfluidic organ-on-a-chip (organ chip) cultures. Here, we describe a new microfluidic organ chip design that contains embedded electrodes, and we demonstrate its utility for assessing formation and disruption of barrier function both within a human lung airway chip lined by a fully differentiated mucociliary human airway epithelium and in a human gut chip lined by intestinal epithelial cells. These chips with integrated electrodes enable real-time, non-invasive monitoring of TEER and can be applied to measure barrier function in virtually any type of cultured cell.
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