Journal
LAB ON A CHIP
Volume 17, Issue 10, Pages 1817-1825Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7lc00363c
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Funding
- Korean Health, Technology R&D Project, Ministry of Health & Welfare, Republic of Korea [HN14C0090, NRF-2015R1A2A1A09005662, NRF-2014M3C7A1074040, 2014M1A8A1049355, NRF-2015M3A9D7051910]
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There are many proposed mechanisms by which single cells can be trapped; among them is the through-hole membrane for the characterization of individual microorganisms. Due to the small scale of the fabricated pores, the construction of through-hole membranes on a large scale and with relatively large areas faces many difficulties. This paper describes novel fabrication methods for a large-area, freestanding micro/nano through-hole membrane constructed from versatile membrane materials using through-hole membranes on a microfluidic chip (THMMC). This process can rapidly (< 20 min) fabricate membranes with high fidelity multiscale hole size without residual layers. The through-hole site was easily customizable from the micro to the nanoscale, with a low or high aspect ratio giving rise to reliable membranes. Also, the rigidity and biocompatibility of the through-hole membrane are easily tunable by simple injection of versatile membrane materials to obtain a large area (up to 3600 mm(2)). Membranes produced in this manner were then applied as a proof of concept for the isolation, cultivation, and quantification of individual micro-algal cells for selection with respect to the growth rate, while controlling the quorum sensing mediated metabolic and proliferative changes.
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