Journal
LAB ON A CHIP
Volume 17, Issue 16, Pages 2861-2872Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7lc00451f
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Funding
- NSF Biomanufacturing IGERT
- NDSEG Fellowship Program
- NSF CCF [1552784]
- NIH [CA163481]
- Direct For Computer & Info Scie & Enginr
- Division of Computing and Communication Foundations [1552784] Funding Source: National Science Foundation
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Studying cell behavior within 3D material niches is key to understanding cell biology in health and diseases, and developing biomaterials for regenerative medicine applications. Current approaches to studying these cell-material niches have low throughput and can only analyze a few replicates per experiment resulting in reduced measurement assurance and analytical power. Here, we report 3D material cytometry (3DMaC), a novel high-throughput method based on microfabricated, shape-specific 3D cell-material niches and imaging cytometry. 3DMaC achieves rapid and highly multiplexed analyses of very high replicate numbers (n of 10(4)-10(6)) of 3D biomaterial constructs. 3DMaC overcomes current limitations of low n, low-throughput, and noisy assays, to provide rapid and simultaneous analyses of potentially hundreds of parameters in 3D biomaterial cultures. The method is demonstrated here for a set of 85000 events containing twelve distinct cell-biomaterial micro-niches along with robust, customized computational methods for high-throughput analytics with potentially unprecedented statistical power.
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