Journal
ANALYTICAL METHODS
Volume 11, Issue 18, Pages 2410-2414Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9ay00461k
Keywords
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Funding
- NSFC of Hunan Province [2018JJ2083]
- Characteristic Application Discipline of Materials Science and Engineering of Hunan Province
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A novel fluorescence signal-on biosensing platform was developed by using photoinduced electron transfer (PET) between copper nanoclusters (CuNCs) and G-quadruplex/hemin. Hairpin DNA was chosen as an effective template to synthesize CuNCs. A split G-quadruplex sequence is linked to the end of the hairpin DNA template. In the presence of K+ and hemin, the split G-quadruplex sequence can form a Gquadruplex/ hemin complex and quench the fluorescence of CuNCs via PET. Target recognition causes split G-quadruplexes to move away from each other, and prevents the formation of a G-quadruplex/ hemin complex. Thus, a significant fluorescence signal increase is observed, because of the lower PET efficiency. This strategy is successfully applied for the detection of DNA. Otherwise, it can be used for developing a platform for detection of small molecules and proteins.
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