Journal
KIDNEY INTERNATIONAL
Volume 92, Issue 2, Pages 397-414Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.kint.2017.02.001
Keywords
aldosterone; distal tubule; hypernatremia; hyponatremia; proximal tubule
Categories
Funding
- APS STRIDE Undergraduate Summer Research Fellowship [1R25HL115473-01]
- Bastyr University Faculty Research Seed Grant
- National Institute of Diabetes and Digestive and Kidney Diseases Grant [1R01DK110621-01]
- O'Brien Center for Acute Kidney Injury Research Grant [P30DK079337]
- Diabetes Endocrinology Research Center [P30DK063491]
- American Heart Association [15BGIA22410018]
- Satellite Healthcare
- Department of Veterans Affairs Merit Review Award
- Center on Genetics of Transport and Epithelial Biology at University of Cincinnati
- Lundbeck Foundation
- Danish Medical Research Council
- Novo Nordisk Foundation
Ask authors/readers for more resources
The sodium/proton exchanger isoform 3 (NHE3) is expressed in the intestine and the kidney, where it facilitates sodium (re) absorption and proton secretion. The importance of NHE3 in the kidney for sodium chloride homeostasis, relative to the intestine, is unknown. Constitutive tubule-specific NHE3 knockout mice (NHE3(loxloxCre)) did not show significant differences compared to control mice in body weight, blood pH or bicarbonate and plasma sodium, potassium, or aldosterone levels. Fluid intake, urinary flow rate, urinary sodium/creatinine, and pH were significantly elevated in NHE3(loxloxCre) mice, while urine osmolality and GFR were significantly lower. Water deprivation revealed a small urinary concentrating defect in NHE3(loxloxCre) mice on a control diet, exaggerated on low sodium chloride. Ten days of low or high sodium chloride diet did not affect plasma sodium in control mice; however, NHE3(loxloxCre) mice were susceptible to low sodium chloride (about -4 mM) or high sodium chloride intake (about D2 mM) versus baseline, effects without differences in plasma aldosterone between groups. Blood pressure was significantly lower in NHE3(loxloxCre) mice and was sodium chloride sensitive. In control mice, the expression of the sodium/phosphate co-transporter Npt2c was sodium chloride sensitive. However, lack of tubular NHE3 blunted Npt2c expression. Alterations in the abundances of sodium/chloride cotransporter and its phosphorylation at threonine 58 as well as the abundances of the alpha-subunit of the epithelial sodium channel, and its cleaved form, were also apparent in NHE3(loxloxCre) mice. Thus, renal NHE3 is required to maintain blood pressure and steady-state plasma sodium levels when dietary sodium chloride intake is modified.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available