Journal
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC
Volume 30, Issue 5, Pages 357-362Publisher
AKADEMIAI KIADO RT
DOI: 10.1556/1006.2017.30.5.4
Keywords
Salvia officinalis L.; Thin-layer chromatography-direct bioautography; Antibacterial activity; Bioassay; Liquid chromatography-quadrupole time-of-flight mass spectrometry
Categories
Funding
- Hungarian Scientific Research Fund (OTKA) [PD83487]
- Bolyai grant
- [POPW.01.03.00-06-003/09-00]
Ask authors/readers for more resources
Thin-layer chromatography-direct bioautography (TLC-DB) followed by liquid chromatography. tandem mass spectrometry (LC. MS/MS) was used for screening and tentative identification of the antibacterial constituents of Salvia officinalis L. ethanol extract. Seven bacterial strains were used as test organisms, both pathogenic and nonpathogenic, that is, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Staphylococcus epidermidis, Micrococcus luteus, Bacillus subtilis, luminescence gene-tagged Pseudomonas syringae pv. maculicola, and naturally luminescent marine bacterium Aliivibrio fischeri. Eight fractions with the widest antimicrobial spectrum were detected using TLC-DB, isolated by semi-preparative TLC, and subjected to LC-MS/MS analyses. Finally, five bioactive components were tentatively identified, based on their fragmentation pattern, such as salvigenin, cirsimaritin, rosmanol, carnosic acid, and 12-O-methyl carnosic acid.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available