Journal
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
Volume 18, Issue 7, Pages 615-625Publisher
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1600555
Keywords
Silver nanoparticles (AgNPs); Dental casting; Cytotoxicity; Antibacterial; MC3T3-E1; BMSC
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Funding
- Public Welfare Projects of Science Technology Department of Zhejiang Province [2013c33139]
- Natural Science Foundation of Zhejiang Province, China [LZ14C200001]
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The aim of this study was to research the changes in cytotoxicity and antibacterial properties after silver nanoparticles (AgNPs) were incorporated into the surface coating of dental alloys. AgNPs were attached to cobalt chromium alloys and pure titanium using a hydrothermal method, according to the reaction: AgNO3+NaBH4 -> Ag+1/2H(2)+1/2B(2)H(6)+NaNO3. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of the alloys when in contact with osteogenic precursor cells (MC3T3-E1) from mice and mesenchymal stem cells (BMSC) from rats. The antibacterial properties of dental alloys incorporating three different concentrations (10, 4, and 2 mu mol/L) of AgNPs were tested on Staphylococcus aureus (SA) and Streptococcus mutans (MS). High cytotoxicity values were observed for all dental alloys that contained 0% of AgNPs (the control groups). The incorporation of AgNPs reduced cytotoxicity values. No significant difference was observed for antibacterial performance when comparing dental alloys containing AgNPs to the respective control groups. The results demonstrated that the cobalt chromium alloys and pure titanium all had cytotoxicity to MC3T3-E1 and BMSC and that the incorporation of AgNPs could reduce this cytotoxicity. The concentrations of AgNPs adopted in this study were found to have no antibacterial action against SA or MS.
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