Journal
JOURNAL OF WATER AND HEALTH
Volume 15, Issue 6, Pages 885-897Publisher
IWA PUBLISHING
DOI: 10.2166/wh.2017.090
Keywords
drinking water; heterotrophic bacteria; identification; MALDI-TOF MS; water treatment
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Funding
- FPU from Spanish Ministry of Education [FPU12/00614]
- Generalitat de Catalunya [SGR-2014-007]
- Spanish Ministry of Science and Innovation [CGL2011-25401]
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The aim of this work was to assess the suitability of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) for routine heterotrophic monitoring in a drinking water treatment plant. Water samples were collected from raw surface water and after different treatments during two campaigns over a 1-year period. Heterotrophic bacteria were studied and isolates were identified by MALDI-TOF MS. Moreover, the diversity index and the coefficient of population similarity were also calculated using biochemical fingerprinting of the populations studied. MALDI-TOF MS enabled us to characterize and detect changes in the bacterial community composition throughout the water treatment plant. Raw water showed a large and diverse population which was slightly modified after initial treatment steps (sand filtration and ultrafiltration). Reverse osmosis had a significant impact on the microbial diversity, while the final chlorination step produced a shift in the composition of the bacterial community. Although MALDI-TOF MS could not identify all the isolates since the available MALDI-TOF MS database does not cover all the bacterial diversity in water, this technique could be used to monitor bacterial changes in drinking water treatment plants by creating a specific protein profile database for tracking purposes.
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