Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 248, Issue -, Pages 26-30Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2017.06.001
Keywords
Rift Valley fever virus; 4-Segmented; Virus neutralization test; GFP; Ruminants
Funding
- Zoonotic Anticipation and Preparedness Initiative (ZAPI) [115760]
- IMI
- European Commission
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Antibodies specific for Rift Valley fever virus (RVFV) can be detected by diverse methods, including ezyme-linked immunosortbent assay (ELISA) and virus neutralization test (VNT). The VNT is superior in sensitivity and specificity and is therefore considered the gold standard serological assay. Classical VNTs make use of virulent RVFV and therefore have to be performed in biosafety level 3 laboratories. Here, we report the development of a novel VNT that is based on an avirulent RVFV expressing the enhanced green fluorescent protein (eGFP), which can be performed safely outside level 3 biocontainment facilities. Evaluation with a broad panel of experimental sera and field sera demonstrated that this novel VNT is faster and more sensitive than the classical VNT.
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