Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 242, Issue -, Pages 53-57Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2017.01.006
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Funding
- German Center for Infectious Diseases (DZIF)
- HRCMM (Heidelberg Research Center for Molecular Medicine) Career Development Fellowship
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Respiratory syncytial virus (RSV) is a common cause of lower respiratory tract infection in young infants and a major cause of nosocomial infection in pediatric care. Currently available RSV point-of-care tests are of limited sensitivity and relatively expensive. We developed and evaluated a novel RSV rapid test for use at point-of-care, based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) for direct testing of nasopharyngeal swab specimens. RT-LAMP can detect RSV within 30 min, without the need for RNA extraction. The sensitivity of our RT-LAMP assay was 70-80% in comparison to RTPCR. The RT-LAMP test sensitivity is at least equivalent to currently available rapid antigen detection tests (RADT), and the cost of RT-LAMP test reagents is only approximately 10% of that of commercially available RADT tests. RT-LAMP appears to be an attractive alternative to RADT, particularly in settings with limited financial resources. Future improvements could include lyophilization of test reagents and automated read-out of RT-LAMP results. (C) 2017 Elsevier B.V. All rights reserved.
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