Encapsulated enhanced silver nanoparticles biosynthesis by modified new route for nano-biocatalytic activity

The study aims to investigate the green synthesis of silver nanoparticles (AgNPs) by Phyllanthus acidus extract (leaf and twig) as reducing and capping agents and evaluation of their bionanocatalytic activities for the first time. The synthesized AgNPs were characterized by UV-Visible spectrophotometry, Fourier Transform Infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS) and Differential Scanning Calorimetry (DSC). The antioxidant ability of plant extract and AgNPs was analyzed using DPPH*, NO* and OH* scavenging assays. SEM and DLS analysis confirmed the morphology of the nanoparticles to be spherical and the average size to be 48.36 and 164.30 nm. The most efficient scavenger was LAgNPs with EC50 of 58.83 +/- 1.65 mu g/ml in DPPH* and TAgNPs in NO* (EC50, 60.75 +/- 1.59 mu g/ml) and OH* (EC50, 43.07 +/- 1.62 mu g/ml) scavenging assays. Further, they have enhanced the enzyme activity of alpha-amylase, cellulase, and xylanase over 2-6 folds. The results suggest that the Phytofabricated AgNPs from P. acidus can be exploited for industrial and biomedical applications.