Journal
CIENCIA E INVESTIGACION AGRARIA
Volume 46, Issue 1, Pages 30-39Publisher
PONTIFICIA UNIV CATOLICA CHILE, FAC AGRONOMIA INGENIERIA FORESTAL
DOI: 10.7764/rcia.v46i1.1955
Keywords
Cultivated; enzyme activity; Kaempferol; naringenin; wild
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Funding
- Graduate Natural Resources Program at the Universidad de La Frontera (Temuco, Chile)
- Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) scholarship [21110939]
- Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) [11140608]
- FONDECYT [1141245, 3180569]
- Proyectos Basales y Vicerrectoria de Investigacion, Desarrollo e Innovacion (Universidad de Santiago de Chile)
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Flavonoid biosynthesis may be affected by plant domestication, with flavonoid production being reduced in proportion to the degree of domestication. In this context, kaempferol (3,4',5,7-tetrahydroxyflavone) has been identified in the leaves of wild and cultivated Ugni molinae, a berry endemic to Chile. The biosynthetic pathway of kaempferol production begins with naringenin (4', 5,7-trihydroxyflavanone), which is converted to dihydrokaempferol (3,4', 5,7-tetrahydroxyflavanone), catalyzed by flavanone 3 beta-hydroxylase (FHT) and then converted to kaempferol by a bifunctional enzyme called flavonol synthase (FLS). Therefore, our study aims to evaluate how FLS activity is affected in murtilla plants that are subjected to the domestication process. Kaempferol was quantified from methanolic extracts of leaf samples collected from both cultivated and wild U. molinae plants using high-performance liquid chromatography, and enzyme extraction was performed to determine FLS activity. The results showed that kaempferol concentration in wild plants from the Soloyo (0.14 mu g g(-1)), Mehuin (0.18 mu g g(-1)) and Queule (0.25 mu g g(-1)) sampling areas was higher than in their cultivated counterparts. Our data are consistent with the FLS activity detected in samples obtained from Manzanal Alto (134.79 pKatal, Soloyo (96.48 pKatal), and Mehuin (119.97 pKatal). These samples also exhibited higher enzymatic activity than their cultivated counterparts. Together, these data suggest that FLS activity is negatively affected by the domestication process.
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