Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 7, Issue 21, Pages 3431-3437Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9tb00175a
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Funding
- NSFC [21502067, 21672083, 21877048]
- Natural Science Foundation of Shandong Province, China [ZR2014BP001]
- Taishan Scholar Foundation [TS 201511041]
- University of Jinan [309-10004, 160082101]
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The development of two-photon bio-imaging probes for trapping specific enzyme activities in overexpressed tissue is very practical and significant. In this work, we developed a two-photon fluorescent b-gal probe G-GAL, which was made with GCTPOC as the fluorescence reporter and a b-galactosidase cleavable crew as the enzyme activity initiator. The purpose of designing this probe was to detect b-galactosidase in living cells and tissues. The probe G-GAL has the advantage of rapid response, great fluorescence enhancement and superior specificity. Furthermore, the probe has excellent tissue penetration.
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