Journal
CURRENT OPINION IN ELECTROCHEMISTRY
Volume 14, Issue -, Pages 53-59Publisher
ELSEVIER
DOI: 10.1016/j.coelec.2018.12.005
Keywords
Electropolymerization; Direct electron transfer; Redox marker; Epitope imprinting; Biomarker
Funding
- Deutsche Forschungsgemeinschaft (DFG, Germany) [EXC 314]
- ERA-Chemistry 2014 [61133]
- NKFIH OTKA (Hungary) [NN117637]
- BME-Nanotechnology (Hungary) FIKP grant of EMMI (BME FIKP-NAT)
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Electrochemical synthesis and signal generation dominate among the almost 1200 articles published annually on protein-imprinted polymers. Such polymers can be easily prepared directly on the electrode surface, and the polymer thickness can be precisely adjusted to the size of the target to enable its free exchange. In this architecture, the molecularly imprinted polymer (MIP) layer represents only one 'separation plate'; thus, the selectivity does not reach the values of 'bulk' measurements. The binding of target proteins can be detected straightforwardly by their modulating effect on the diffusional permeability of a redox marker through the thin MIP films. However, this generates an 'overall apparent' signal, which may include nonspecific interactions in the polymer layer and at the electrode surface. Certain targets, such as enzymes or redox active proteins, enables a more specific direct quantification of their binding to MIPs by in situ determination of the enzyme activity or direct electron transfer, respectively.
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