Journal
DYES AND PIGMENTS
Volume 120, Issue -, Pages 208-212Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2015.04.013
Keywords
Rhodamine; FRET; Fluorescent probe; Hg2+; Living cells; Aqueous solution
Funding
- National Natural Science Foundation of China [J1210064]
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A coumarin rhodamine based ratiometric fluorescent probe was designed and successfully synthesized. The probe can detect Hg2+ with high selectivity over other metal ions and high sensitivity with the most sensitive readout signal in aqueous solution. There is a good linear relationship between the fluorescence ratio I-587/I-478 and the concentration of Hg2+ ranging from 0.5 uM to 1.5 uM and the detection limit was calculated to be as low as 3.2 nM. In addition, the probe can quicldy detect over a wide pH range from 4 to 11. Furthermore, the probe is cell membrane permeable and can detect Hg2+ in living cells. (C) 2015 Elsevier Ltd. All rights reserved.
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