Journal
NEW JOURNAL OF CHEMISTRY
Volume 43, Issue 23, Pages 9076-9083Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9nj01101c
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Funding
- Natural Science Foundation of Hebei Province [B2017203229]
- Youth Foundation Project - Hebei Education Department of China [QN2015034]
- China Postdoctoral Science Foundation [2016M601284]
- Postdoctoral Science Foundation of Hebei Province [B2016003017]
- National Nature Science Foundation of China [21474085, 21674092]
- National Development Project on Key Basic Research (973 Project) [2015CB655303]
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The development of ultra-small platinum nanoparticles is very important for the sensitive detection of glucose in complex medium without protein interference. Zwitterionic dendrimer-encapsulated platinum nanoparticles (Pt-n-G5MC NPs) have been developed and studied for the highly sensitive and selective colorimetric detection of glucose. Firstly, zwitterionic dendrimer (G5MC) was obtained by surface modification of generation 5 poly(amidoamine) dendrimer with maleic anhydride and cysteamine. The calculated average diameters of Pt nanoparticles (Pt NPs) for Pt-n-G5MC (n = 55, 110, and 165) were 1.64 +/- 0.22, 2.09 +/- 0.24, and 2.98 +/- 0.29 nm, respectively. Pt-n-G5MC NPs not only had high stability in various pH solutions and fibrinogen solutions, but also showed no noticeable cytotoxicity against HUVEC cells and HeLa cells. In addition, Pt-n-G5MC NPs catalyzed oxidation of 3,3,5,5-tetramethylbenzidine (TMB) with H2O2 to generate blue oxidized TMB (oxTMB), indicating their peroxidase-like property. This peroxidase-like reaction followed a typical Michaelis-Menten kinetics. Furthermore, this colorimetric reaction was used for glucose detection with high sensitivity and selectivity. The linear range of glucose concentration with absorbance at 652 nm was from 7 to 1380 M, and the detection limit was 2.8 M. More importantly, the relative activity of Pt-n-G5MC NPs was not affected by proteins. These enhanced properties are attributed to the ultra-small size of Pt NPs and high stability of Pt-55-G5MC NPs in complex medium. The glucose concentration in saliva as a real sample was found to be 0.18 mM. The proposed glucose sensor could have promising applications for glucose detection in biocatalysis and bioassays in the future.
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