Journal
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Volume 97, Issue 12, Pages 4204-4209Publisher
WILEY
DOI: 10.1002/jsfa.8294
Keywords
immunomodulatory capacity; surface-layer protein; purification; Lactobacillus acidophilus
Funding
- Natural Science Foundation of China [31471598, 31571852, 31601487]
- Natural Science Foundation of Jiangsu Province [BK20141447, BK20151544]
- Natural Science Funding of Zhejiang [LQ16C200002]
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BACKGROUNDSurface-layer proteins (SLP) have been found in the outermost layer of the cell wall in many types of lactobacillus are considered to be an important factor with respect to intestinal immunity. RESULTSThe present study compared the effects of SLP extracted by different concentrations of LiCl and carbamide, and subsequently identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, circular dichroism and differential scanning calorimetry. Furthermore, RAW 264.7 cells were used to evaluate the immunomodulatory effects of SLP. SLP were derived from Lactobacillus acidophilusCICC6074 with a molecular weight of 46kDa, and consisted of 16.9% -helix, 42.3% -sheet, 20.8% -turns and 22.5% random coils. SLP promoted NO secretion and higher quantities of NO were produced as the SLP concentrations increased. SLP concentrations over 50 mu gmL(-1) significantly decreased the amount of tumor necrosis factor- secreted by RAW264.7 cells. CONCLUSIONSLP can trigger immunomodulatory effects in RAW 264.7 cells. This provides crucial information that will enable the further use of L.acidophilus in food, medicine and other products. (c) 2017 Society of Chemical Industry
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