Journal
SN APPLIED SCIENCES
Volume 1, Issue 6, Pages -Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1007/s42452-019-0581-6
Keywords
Graphene oxide; Alginate; Nanocomposite; Bone scaffold; Cytocompatibility; Bone tissue engineering
Categories
Funding
- National Natural Science Foundation of China [31700840]
- Key Scientific Research Project of Henan Province [18B430013]
- Scientific Research Foundation of Graduate School of Xinyang Normal University [2018KYJJ48]
- Wuhan Municipal Health and Family Planning Research Fund [WX18C23]
- Nanhu Scholars Program for Young Scholars of XYNU
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Porous graphene oxide (GO) nanocomposite as scaffold has attracted increasing attention in bone tissue engineering recently. In this study, GO nanosheets was modified by 2,2'-(ethylenedioxy)-diethnethiol (EDDET), and dithiol-modified GO (DT-GO) nanosheets were obtained. The results confirmed that GO nanosheets were cross-linked by EDDET, meanwhile, the distance between GO layers reduced after modification. Next, DT-GO nanosheets were further incorporated into alginate hydrogels to fabricate DT-GO/alginate (DT-GA) nanocomposite as scaffold. The prepared DT-GA nanocomposite behaved the laminar network morphology with interconnected porous structure confirmed by SEM analysis. In addition, the DT-GA nanocomposite with the concentration of DT-GO nanosheets at 16.7% showed the highest porosity value and lowest compressive strength. Furthermore, the bone marrow derived mesenchymal stem cells (BMSCs) showed a good proliferation on DT-GA nanocomposite, demonstrated that prepared nanocomposite had a good cytocompatibility, which was identified by CCK-8 assay and fluorescent microscopy images. Lastly, ALP activity analysis certified that BMSCs seeded on DT-GA nanocomposite could differentiate into osteoblastic phenotype. Above results suggest that the dithiol-modified GO/alginate nanocomposite has the potential to be applied in bone tissue engineering.
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