Journal
LAB ON A CHIP
Volume 19, Issue 14, Pages 2435-2443Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9lc00163h
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Funding
- Harvard Materials Research Science and Engineering Center, National Science Foundation [DMR-1420570]
- National Science Foundation under NSF [1541959]
- Cytonome/ST, LLC (Bedford, Massachusetts)
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We report a microfluidic fluorescence activated cell-sorting (mu FACS) device that employs traveling surface acoustic waves (TSAW) to sort cells at rates comparable to conventional jet-in-air FACS machines, with high purity and viability. The device combines inertial flow focusing and sheath flow to align and evenly space cells, improving the sorting accuracy and screening rate. We sort with an interdigital transducer (IDT) whose tapered geometry allows precise positioning of the TSAW for optimal cell sorting. We sort three different cell lines at several kHz, at cell velocities exceeding one meter per second, while maintaining both sorting purity and cell viability at around 90% simultaneously.
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