Journal
ANALYST
Volume 144, Issue 11, Pages 3668-3675Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9an00181f
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Funding
- National Natural Science Foundation of China [21375063]
- Natural Science Foundation of Jiangsu Province [BK20181382]
- Jiangsu Provincial Medical Youth Talent [QNRC2016066]
- Jiangsu Collaborative Innovation Center of Biomedical Functional Materials
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Exosomes are non-invasive biomarkers for cancer diagnosis. Herein, we describe an electrochemiluminescent (ECL) aptasensor for the detection of exosomes from breast tumor cells. Mercaptopropionic acid (MPA)-modified Eu3+-doped CdS nanocrystals (MPA-CdS:Eu NCs) and H2O2 were used as ECL emitters and coreactant, respectively. The exosomes are recognized and captured by the CD63 aptamer, and then form a G-quadruplex/hemin DNAzyme, which efficiently catalyzes the decomposition of H2O2, resulting in the decreased ECL signal of MPA-CdS: Eu NCs. The exosomes from breast tumor cells (MCF-7 cells) can be detected in the range of 3.4 x 10(5) to 1.7 x 10(8) particles per mL. The limit of detection (LOD) was estimated to be 7.41 x 10(4) particles per mL at a signal-to-noise ratio of 3. The aptasensor has been successfully used to detect exosomes in the serum.
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