Journal
NANOSCALE
Volume 11, Issue 28, Pages 13243-13248Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9nr02512j
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Funding
- Project TRoMBONE under the Horizon 2020 program of European commission [748583]
- FCT project [POCI-01-0145-FEDER-029919]
- EC project ERAatUC [669088]
- Marie Curie Actions (MSCA) [748583] Funding Source: Marie Curie Actions (MSCA)
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Here we report a two-step surface modification methodology to radiolabel small extracellular vesicles (SEVs) with (CuCl2)-Cu-64 for PET/MRI imaging. The modification did not change or damage the morphology, surface receptor proteins and internal RNA content. Radiolabeled SEVs could be detected in organs with low accumulation such as the brain (0.4-0.5% ID g(-1)) and their brain location determined by MRI.
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