Journal
BIOFABRICATION
Volume 11, Issue 4, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1758-5090/ab2aa1
Keywords
glycosaminoglycan hydrogels; inkjet printing; cell-instructive materials; tissue engineering
Funding
- European Union Seventh Framework Programme (FP7/2007-2013) [309962]
- Deutsche Forschungsgemeinschaft, project 'Cellinstructive hydrogel gradient arrays' [ZI 1238/4-1, FR 3367/2-1]
- Federal Ministry of Education and Research of Germany [FKZ: 13N13859]
Ask authors/readers for more resources
Materials capable of directing cell fate by providing spatially-graded mechanical and biomolecular cues are critically important in the reconstitution of living matter. Herein, we report a multi-component inkjet bioprinting method that allows for spatially varying composition and network properties in cell-instructive glycosaminoglycan (GAG)-based biohybrid and pure poly(ethylene glycol) hydrogels with unprecedented (50 mu m) resolution. The principle relies on the covalent crosslinking of different polymeric precursors through a very rapid bio-orthogonal Michael type addition scheme adjusted in ways to occur during the fusion ofbio-ink droplets prior to and upon contact with the target. Exemplary data show that chemotactic molecular gradients produced by this approach within printed GAG-gels of defined zonal architecture can effectively direct migratory activity and morphogenesis of embedded human bone-marrow derived mesenchymal stem cells. The introduced methodology is expected to enable a new, holistic level of control over reductionistic tissue and organoid models.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available