Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 28, Issue 10, Pages 2099-2107Publisher
SPRINGER
DOI: 10.1007/s13361-017-1740-x
Keywords
Mass spectrometry imaging; IR-MALDESI; Tissue imaging; Spatial resolution; Laser focusing
Funding
- National Institutes of Health [R01GM087964]
- W.M. Keck Foundation
- North Carolina State University
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High spatial resolution in mass spectrometry imaging (MSI) is crucial to understanding the biology dictated by molecular distributions in complex tissue systems. Here, we present MSI using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) at 50 mu m resolution. An adjustable iris, beam expander, and an aspherical focusing lens were used to reduce tissue ablation diameters for MSI at high resolution. The laser beam caustic was modeled using laser ablation paper to calculate relevant laser beam characteristics. The minimum laser spot diameter on the tissue was determined using tissue staining and microscopy. Finally, the newly constructed optical system was used to image hen ovarian tissue with and without oversampling, detailing tissue features at 50 mu m resolution.
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