Journal
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
Volume 21, Issue 6, Pages 583-593Publisher
BEGELL HOUSE INC
DOI: 10.1615/IntJMedMushrooms.2019030857
Keywords
apoptosis; antioxidant; Inonotus obliquus; medicinal mushrooms; mitochondrial pathway; tacrine
Funding
- Liaoning Provincial Science Program [20180530002]
- National Key Research and Development Program of China [2017YFC1702006]
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Tacrine is the first drug licensed for the treatment of Alzheimer disease. Unfortunately, reversible hepatotoxicity limits its clinical use. In our previous study, we found that tacrine induced apoptosis in HepG2 cells by reactive oxygen species (ROS) formation and mitochondria dysfunction. lnonotus obliquus is a mushroom traditionally used as a folk medicine in Asia. In this study, the possible protective effect of polysaccharides from I. obliquus was investigated. The results showed that I. obliquus polysaccharides (IOP) reduced tacrine-induced apoptosis in HepG2 cells. Inhibition of tacrine-induced ROS generation, 8-OHdG formation in mitochondrial DNA, and loss of the mitochondrial transmembrane potential by IOP were also observed. Furthermore, IOP decreased the cytochrome c release and activation of caspase-3 induced by tacrine. These data suggest that IOP could inhibit tacrine-induced apoptosis in HepG2 cells. The protection is mediated by an antioxidant protective mechanism. Consumption of IOP may be a plausible way to prevent tacrine-induced hepatotoxicity.
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