Journal
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
Volume 65, Issue 3, Pages 121-128Publisher
MICROBIOL RES FOUNDATION
DOI: 10.2323/jgam.2018.07.002
Keywords
beta-carotene; carotenoids; crtYB; Sporidiobolus pararoseus; transcriptome
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Funding
- National Natural Science Foundation of China [31271818]
- National Key Research and Development Program of China [2017YFD200807-3]
- Natural Science Foundation of Liaoning Province of China [2015020763]
- Shenyang Science and Technology Innovation in Agriculture Special Fund [F17-150-3-00]
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Carotenoids are ubiquitous constituents of living organisms. These structurally diverse pigments have received considerable attention due to their biotechnological applications and potential beneficial effects on human health. In this study, we characterized an over-producing beta-carotene mutant of Sporidiobolus pararoseus, obtained by ultraviolet mutagenesis, named MuY9. We compared the transcriptome between the wild-type and MuY9. A total of 348 differential expressed genes (DEGs) were found, and only one DEG crtYB is involved in carotenoid biosynthesis. The overproduction of beta-carotene in MuY9 should be attributed to the upregulation of crtYB. Functional identification of crtYB was performed using heterologous complementation in Escherichia coli. Our findings indicate that the enzymatic conversions of geranylgeranyl pyrophosphate to phytoene, as well as lycopene to beta-carotene, are catalyzed by this CrtYB. Furthermore, our insights into the crtYB gene should facilitate a more detailed understanding of the carotenogenic pathway in S. pararoseus, and advance the development of the genetic engineering for the bio-production of carotenoids.
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