Journal
BRIEFINGS IN BIOINFORMATICS
Volume 20, Issue 3, Pages 866-876Publisher
OXFORD UNIV PRESS
DOI: 10.1093/bib/bbx147
Keywords
de novo assembly; third-generation sequencing; single-molecule sequencing; PacBio SMRT; assembly evaluation
Funding
- JSPS KAKENHI [16H06279]
- Grants-in-Aid for Scientific Research [16H06279] Funding Source: KAKEN
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Long reads obtained from third-generation sequencing platforms can help overcome the long-standing challenge of the de novo assembly of sequences for the genomic analysis of non-model eukaryotic organisms. Numerous long-read-aided de novo assemblies have been published recently, which exhibited superior quality of the assembled genomes in comparison with those achieved using earlier second-generation sequencing technologies. Evaluating assemblies is important in guiding the appropriate choice for specific research needs. In this study, we evaluated 10 long-read assemblers using a variety of metrics on Pacific Biosciences (PacBio) data sets from different taxonomic categories with considerable differences in genome size. The results allowed us to narrow down the list to a few assemblers that can be effectively applied to eukaryotic assembly projects. Moreover, we highlight how best to use limited genomic resources for effectively evaluating the genome assemblies of non-model organisms.
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