Journal
EUROPEAN CELLS & MATERIALS
Volume 37, Issue -, Pages 292-309Publisher
AO RESEARCH INSTITUTE DAVOS-ARI
DOI: 10.22203/eCM.v037a18
Keywords
Bone; multipotent stromal cells; osteogenesis; repair; collagen membrane; guided bone regeneration
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Funding
- Geistlich Pharma, Wolhusen, Switzerland [101930]
- AO Foundation [S-16-132E]
- EPSRC Centre for Doctoral Training in Tissue Engineering and Regenerative Medicine Innovation in Medical and Biological Engineering multidisciplinary collaboration of Faculties at the University of Leeds [EP/L014823/1]
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Barrier membranes are popularly used for guided bone regeneration (GBR). However, more knowledge is needed to assess how these membranes could be of therapeutic value when populated with native multipotent stromal cells (MSCs), particularly in the orthopaedic field. The present manuscript investigated the activities of human bone marrow-multipotent stromal cells (BM-MSCs) when loaded on two differently structured pure collagen membranes. A crosslinked collagen membrane (CS) was tested versus a non-crosslinked bilayer collagen membrane, Bio-Gide (R) (BG). Following loading with BM aspirate containing native MSCs, cell attachment to the membranes was examined using electron microscopy and flow cytometry. Furthermore, alkaline phosphatase (ALP) expression and calcium deposition levels were investigated for these BM-aspirate-loaded membranes. Culture-expanded BM-MSCs were also used to load membranes and confirm the MSC functional data. All membranes supported BM-MSC attachment. However, larger numbers of attached BM-MSCs were detected for CS as compared to BG (p = 0.0010). In osteogenic medium, ALP activity was higher for CS than BG (p = 0.0312). Total calcium deposition (not normalised to cell count) was also higher for CS than BG (p = 0.0073). Consistently, the normalised secreted vascular endothelial growth factor A (VEGF-A) levels were higher in BM-MSCs loaded on CS relative to BG (p = 0.0302). Collectively, both collagen membranes supported the osteogenic functions of BM-MSCs. However, CS was found to be overall superior, probably since it provided more BM-MSC attachment. These collagen membranes could potentially be used to improve GBR outcomes in orthopaedic applications.
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