Journal
CHEMICAL COMMUNICATIONS
Volume 55, Issue 61, Pages 8963-8966Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9cc04369a
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Funding
- National Natural Science Foundation of China [21527811, 21735003, 21705096]
- Award for Team Leader Program of Taishan Scholars of Shandong Province, China
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We develop a simple method for sensitive detection of alkaline phosphatase (ALP) based on the ligase amplification reaction-catalyzed assembly of a single quantum dot (QD)-based nanosensor. This nanosensor requires only a single ligase enzyme to achieve ultrahigh sensitivity with a detection limit of 5.63 x 10(-7) U mL(-1), and can be applied for kinetic analysis, inhibitor screening, and ALP measurement in cell extracts.
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