Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 139, Issue 11, Pages 3920-3929Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.6b12822
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Funding
- National Institutes of Health [P30 DA018310]
- National Institute on Drug Abuse [U01 MH109062]
- National Institute of Mental Health
- National Science Foundation [CHE 16-067915]
- NSF Graduate Research Fellowship Program
- Springborn Fellowship
- Training Program at Chemistry-Interface with Biology [T32 GM070421]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1606791] Funding Source: National Science Foundation
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The chemical differences between individual cells within large cellular populations provide unique information on organisms' homeostasis and the development of diseased states. Even genetically identical cell lineages diverge due to local microenvironments and stochastic processes. The minute sample volumes and low abundance of some constituents in cells hinder our understanding of cellular heterogeneity. Although amplification methods facilitate single-cell genomics and transcriptomics, the characterization of metabolites and proteins remains challenging both because of the lack of effective amplification approaches and the-wide diversity in cellular constituents. Mass spectrometry has become, an enabling technology for the investigation of individual cellular metabolite profiles with its exquisite sensitivity, large dynamic range, and ability to characterize hundreds to thousands of compounds. While advances in instrumentation have improved figures of merit, acquiring measurements at high throughput and sampling from large populations of cells are still not routine. In this Perspective, we highlight the current trends and progress in mass-spectrometry-based analysis of single cells, with a focus on the technologies that will enable the next generation of single-cell measurements.
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