Journal
ADVANCED BIOSYSTEMS
Volume 3, Issue 9, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adbi.201900061
Keywords
cryo-scanning electron microscopy; nanoparticles; sperm cells; surface charge; zetasizer
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Funding
- Zukunftskonzept of the TU Dresden under the Excellence Initiative of the German Science Foundation (DFG)
- Volkswagen Foundation [91619]
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In this article, a procedure to investigate sperm charge distribution by electrostatic sperm-particle interactions is demonstrated. Differently chargedparticles are fabricated and their attachment distribution on the bovine sperm membrane is investigated. The sperm-particle attachment sites are observed using bright field and cryo-scanning electron microscopy combined with energy-dispersive X-ray analysis. The findings suggest that the charge distribution of the sperm membrane is not uniform, and although the overall net charge of the sperm cell is negative, positively charged areas are especially found on the sperm heads. The newly developed method is used to investigate the dynamic charge distribution of the sperm cell membrane upon maturation induced by heparin, as a representation of the multitude of changes during the development of a sperm.
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