Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 139, Issue 27, Pages 9100-9103Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.7b02145
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Funding
- Charles E. Kaufman Foundation of The Pittsburgh Foundation
- National Science Foundation [MCB-1330746]
- National Institutes of Health [R01GM112728]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1330746] Funding Source: National Science Foundation
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Site-specific incorporation of unnatural amino acids into proteins provides a powerful tool to study protein function. Here we report genetic code expansion in zebrafish embryos and its application to the optogenetic control of cell signaling. We genetically encoded four unnatural amino acids with a diverse set of functional groups, which included a photocaged lysine that was applied to the light-activation of luciferase and kinase activity. This approach enables-versatile manipulation of protein function in live zebrafish embryos, a transparent and commonly used model organism to Study embryonic development.
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