Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 139, Issue 37, Pages 13055-13062Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.7b06476
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Funding
- National Science Foundation [CHE-1416116]
- Ecole Normale Superieure
- CNRS
- University Pierre and Marie Curie [UMR 8640]
- LIA CNRS NanoBioCatEchem
- ANR grant ChemCatNano-Tech [ANR-AAP-CE06]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1416116] Funding Source: National Science Foundation
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The production of reactive oxygen and nitrogen species (ROS and RNS) in human cells is implicated in various diseases, including cancer. Micrometer-sized electrodes coated with Pt black and platinized Pt nanoelectrodes have previously been used for the detection of primary ROS and RNS in biological systems. In this Article, we report the development of platinized carbon nanoelectrodes with well-characterized geometry and use them as scanning electrochemical microscopy (SECM) tips to measure ROS and RNS inside noncancerous and Metastatic human breast cells. By performing time-dependent quantitative amperometric measurements at different potentials, the relative concentrations of four key ROS/RNS in the cell cytoplasm and their dynamics were determined and used to elucidate the chemical origins and production rates of ROS/RNS in nontransformed and metastatic human breast cells.
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