Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 139, Issue 39, Pages 13830-13836Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.7b07374
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Funding
- National Institutes of Health, a National Science Foundation [GM119707, GM113106, DGE1255832]
- NIH [GM113389]
- DOE Office of Science [DE-AC02-06CH11357]
- Michigan Economic Development Corporation
- Michigan Technology Tri-Corridor [085P1000817]
- National Cancer Institute [ACB-12002]
- National Institute of General Medical Sciences [AGM-12006]
- NIH-Office of Research Infrastructure Programs, High-End Instrumentation Grant [1S100D012289-01A1]
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Tron(II)- and 2-(oxo)-glutarate-dependent oxygenases catalyze diverse oxidative transformations that are often initiated by abstraction of hydrogen from carbon by iron(IV)-oxo (ferryl) complexes. Control of the relative orientation of the substrate: C-H and ferry Fe-O bonds, primarily by direction of the oxo group into One of two cis related coordination sites (termed inline and offline), may be generally important for control of the reaction outcome. Neither the ferryl complexes nor their fleeting precursors have been, crystallograpincally characterized, hindering direct experimental validation of the offline hypothesis and elucidation of the means by which the protein might dictate an alternative oxo position. Comparisori of high-resolution X-ray crystal structures of the substrate complex, an Fe(11)-peroxysuccinate ferryl precursor, and a vanadiurn(IV)-oxo mimic of the ferryl intermediate in the L-arginine 3-hydroxylase, VioC, reveals Coordinated motions of active site residues that appear to control the intermediate geometries to-determine reaction outcome.
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