Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 139, Issue 44, Pages 15907-15913Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.7b08772
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Funding
- EPSRC/University of Sheffield Doctoral Fellowship
- University of Sheffield
- KRG
- Marie Curie Career Integration Grant NanodynacTCELLvation [PCIG13-GA-2013-618914]
- BBSRC [BB/L014327/1] Funding Source: UKRI
- MRC [MR/K015753/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L014327/1] Funding Source: researchfish
- Medical Research Council [MR/K015753/1] Funding Source: researchfish
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Detailed studies on the live cell uptake properties of a dinuclear membrane-permeable Ru-II cell probe show that, at low concentrations, the complex localizes and images mitochondria. At concentrations above similar to 20 mu M, the complex images nuclear DNA. Because the complex is extremely photostable, has a large Stokes shift, and displays intrinsic subcellular targeting, its compatibility with super-resolution techniques was investigated. It was found to be very well suited to image mitochondria and nuclear chromatin in two color, 2C-SIM, and STED and 3D-STED, both in fixed and live cells. hi particular, due to its vastly improved photostability compared to that of conventional SR probes, it can provide images of nuclear DNA at unprecedented resolution.
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