Journal
INORGANICS
Volume 7, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/inorganics7070085
Keywords
urease maturation; metallochaperone; nickel; G-protein; conformational change
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Funding
- Research Grants Council of Hong Kong [14117314, AoE/M-05/12, AoE/M-403/16]
- Research Committee of The Chinese University of Hong Kong [3132814, 3132815]
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Maturation of urease involves post-translational insertion of nickel ions to form an active site with a carbamylated lysine ligand and is assisted by urease accessory proteins UreD, UreE, UreF and UreG. Here, we review our current understandings on how these urease accessory proteins facilitate the urease maturation. The urease maturation pathway involves the transfer of Ni2+ from UreE -> UreG -> UreF/UreD -> urease. To avoid the release of the toxic metal to the cytoplasm, Ni2+ is transferred from one urease accessory protein to another through specific protein-protein interactions. One central theme depicts the role of guanosine triphosphate (GTP) binding/hydrolysis in regulating the binding/release of nickel ions and the formation of the protein complexes. The urease and [NiFe]-hydrogenase maturation pathways cross-talk with each other as UreE receives Ni2+ from hydrogenase maturation factor HypA. Finally, the druggability of the urease maturation pathway is reviewed.
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