Journal
RSC ADVANCES
Volume 9, Issue 41, Pages 23973-23978Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9ra04228h
Keywords
-
Categories
Funding
- Tokyo Tech World Research Hub Initiative (WRHI) Program of the Institute of Innovative Research, Tokyo Institute of Technology [15KK0184, 17H02202, 17K20095, 18H045420]
- Ministry of Education, Culture, Sports, Science and Technology, Japan
Ask authors/readers for more resources
Raman spectroscopic measurements and theoretical calculation revealed that the Raman bands corresponding to the B-H stretching vibrations of two types of simple icosahedral boron clusters, ortho-carborane 3 and closo-dodecaborate 4 appeared at approximately 2450-2700 cm(-1), and did not overlap with those of cellular components. Although ortho-carborane 3 possesses a possible property as a Raman probe, it was difficult to measure Raman imaging in the cell due to its poor water solubility. In fact, ortho-carborane derivative 6, which internally has an alkyne moiety, exhibited very weak Raman signals of the CC stretching and the B-H stretching vibrations were barely detected at a 400 ppm boron concentration in HeLa cells. In contrast, closo-dodecaborate derivatives such as BSH (5) were found to be a potential Raman imaging probe cluster for target molecules in the cell. BSH-conjugated cholesterol 7 (BSH-Chol) was synthesized and used in Raman imaging in cells. Raman imaging and spectral analysis revealed that BSH-based Raman tags provide a versatile platform for quantitative Raman imaging.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available