Journal
PLANT PATHOLOGY JOURNAL
Volume 35, Issue 4, Pages 389-392Publisher
KOREAN SOC PLANT PATHOLOGY
DOI: 10.5423/PPJ.NT.12.2018.0306
Keywords
phylogeny; restriction analysis; sequencing; Strawberry mottle virus
Categories
Funding
- Polish Ministry of Agriculture and Rural Development
Ask authors/readers for more resources
The studies on detection of the Strawberry mottle virus (SMoV) have been conducted in Poland for breeding programme purpose and for producers of strawberry plant material. Leaf samples collected from infected strawberry plants were grafted on Fragaria sp. Indicators which were maintained in greenhouse for further study. Seven Fragaria vesca var. semperflorens 'Alpine' indicators infected by SMoV were used for the study aimed on molecular characterization of virus isolates. Partial RNA2 was amplified from total nucleic acids using the RT-PCR method. The obtained amplicons separately digested with Bfal, Fan', Haan, Hind, and Taql enzymes showed different restriction profiles. The nucleotide sequences analysis of RNA2 fragment confirmed the genetic diversity of the SMoV isolates as their similarity ranged from 94.7 to 100%. Polish isolates shared 75.7-99.2% identity with sequence of the virus strains from the Czech Republic, the Netherlands, and Canada. Phylogenetic analysis resulted in grouping of the isolates found in Poland together with one of the Czech strain whereas two other from the Czech and the strains from the Netherlands and Canada created the separate cluster.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available