Journal
BMC RESEARCH NOTES
Volume 12, Issue 1, Pages -Publisher
SPRINGERNATURE
DOI: 10.1186/s13104-019-4580-x
Keywords
Liquid chromatography tandem mass-spectrometry; LC-MS; MS; Secondary metabolites; Lichen; Usnic acid; Cladonia uncialis
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Funding
- NSERC DG
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Objective The purpose of this study was to develop and validate a specific and sensitive liquid chromatography tandem mass-spectrometry method for quantification of usnic acid concentration in the lichen, Cladonia uncialis, suitable for detection of relatively small fluctuations of usnic acid concentration in response to environmental changes. Results The resulting method was fully validated according to international guidelines and demonstrated good selectivity and sensitivity with minor levels of a matrix effect and high accuracy.
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