Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 198, Issue 3, Pages 163-176Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2017.02.003
Keywords
Helical symmetry; Single-particle analysis; Cryo-electron microscopy; Image processing; Regularised likelihood optimisation
Funding
- UK Medical Research Council [MC_UP_A025_1013]
- MRC [MC_UP_A025_1013] Funding Source: UKRI
- Medical Research Council [MC_UP_A025_1013] Funding Source: researchfish
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We describe a new implementation for the reconstruction of helical assemblies in the empirical Bayesian framework of RELION. Our approach calculates optimal linear filters for the 3D reconstruction by embedding helical symmetry operators in Fourier-space, and deals with deviations from perfect helical symmetry through Gaussian-shaped priors on the orientations of individual segments. By incorporating our approach into the standard pipeline for single-particle analysis in RELION, our implementation aims to be easily accessible for non-experienced users. Although our implementation does not solve the problem that grossly incorrect structures can be obtained when the wrong helical symmetry is imposed, we show for four different test cases that it is capable of reconstructing structures to near-atomic resolution. (C) 2017 MRC Laboratory of Molecular Biology. Published by Elsevier Inc.
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