Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 197, Issue 2, Pages 181-190Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2016.06.024
Keywords
Electron cryo-tomography; Cellular architecture; Sub-volume averaging; Microtubules; Cytoskeleton; Dynein; Adenovirus; Electron cryo-microscopy; Virus trafficking; Viral entry; Retrograde transport; Endocytosis; in situ structure determination
Funding
- Human Frontiers Science Programme (HFSP) [RGP0055/2015]
- Wellcome Trust [107806/Z/15/Z, 060208/Z/00/Z, 093305/Z/10/Z, 090532/Z/09/Z, 099683/Z/12/Z]
- Leverhulme Trust [RPG-2012-519]
- Wellcome Trust [099683/Z/12/Z] Funding Source: Wellcome Trust
Ask authors/readers for more resources
Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms offers a route to increase the signal-to-noise of repetitive biological structures, such improving the information content and interpretability of tomograms. We discuss the potential for sub-volume averaging in highlighting and investigating specific processes in situ, focusing on microtubule structure and viral infection. We show that (i) in situ sub-volume averaging from single tomograms can guide and complement segmentation of biological features, (ii) the in situ determination of the structure of individual viruses is possible as they infect a cell, and (iii) novel, transient processes can be imaged with high levels of detail. (C) 2016 The Authors. Published by Elsevier Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available