Journal
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
Volume 16, Issue 8, Pages 1142-1148Publisher
IVYSPRING INT PUBL
DOI: 10.7150/ijms.34541
Keywords
Gastric cancer; CDK12; lymph node metastasis; CD8(+) cells; CCL21
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Funding
- National Science Foundation of China [81672327, 81802319, 81602411]
- Program of Shanghai Academic/Technology Research Leader [17XD1402600]
- Program for Outstanding Medical Academic Leader [20161410]
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support [20161410]
- Development Grant for Clinical Trial [SHDC12017X06]
- Science and Technology Comission of Shanghai Municipality [STCSM18411953000]
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Background: The aims of this study were to investigate the expression pattern of CDK12 protein in gastric cancer, and to analyze the correlations of CDK12 expression between CD8(+) cell density and CCL12 expression. Methods: Eighty-six paired tumor and non-tumor samples were collected from patients who underwent radical surgery and had pathological confirmed gastric adenocarcinoma. Immunohistochemistry was used to assess CDK12 expression and CD8(+) cell density. Expression of CDK12 and CCL21 mRNA was detected by quantitative reverse transcription-polymerase chain reaction. Results: CDK12 expression in gastric tumor tissues was significantly higher than it in paired non-tumor tissues (P<0.001). High expression of CDK12 was identified in 43 cases (50%), and it was significantly correlated with Lauren's classification (diffuse type) and number of metastatic lymph nodes (>= 15). High CDK12 protein level indicated a relative poorer overall survival than patients with CKD12 low expression, while it was not identified as an independent prognostic factor. Median number of CD8(+) cells in tumor tissues was 51 (range: 0-292). Number of CD8(+) cells was positively correlated with CDK12 expression score in tumor tissues (r=0.243, P=0.024). Positive correlation was also found between CDK12 and CCL21 mRNA expression (r=0.419, P=0.017). Conclusion: High CDK12 expression was detected in gastric cancer which was correlated with malignant phenotypes and worse outcome. Positive correlations of CD8(+) cell number and CCL21 mRNA expression with CDK12 level were identified.
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